Value of 3D printed polyether ether ketone prosthesis in the treatment of scapular tumors / 中华骨科杂志

Polyetheretherketone (PEEK) as a new type of thermoplastic engineering plastic, has good biological activity, elastic modulus close to human cortical bone and radiation permeability, and has been widely used in medical field. This study aims to explore the safety and clinical efficacy of using 3D printing personalized PEEK materials to repaire scapular bone defects after bone tumor resection. A total of 6 patients who underwent the implantation of 3D printed PEEK scapular prosthesis from January 2020 to December 2021 in Yunnan Cancer Hospital were retrospectively analyzed. There were 3 males and 3 females, with age ranged from 14 to 52 years. There were 1 case of synovial sarcoma, 1 of Ewing's sarcoma, and 4 of chondrosarcoma. PEEK prosthesis was designed and fabricated based on CT data before surgery. Tumor resection and prosthesis replacement were performed under the premise of ensuring safe surgical boundaries, including 2 cases of total scapular prosthesis replacement and 4 cases of partial scapular prosthesis replacement. The operation time was 90-170 min, and the intraoperative blood loss was 100-400 ml. All 6 patients received satisfactory follow-up, with a tumor progression free survival time of 16-28 months. No tumor recurrence or metastasis was observed, and all patients survived tumor free. At last follow-up, the Constant-Murley shoulder joint score was a minimum of 62 points and a maximum of 68 points. The Japanese Orthopaedic Association's shoulder joint score was 63 points minimum and 78 points maximum. Computer-aided design 3D printing PEEK material prosthesis has certain advantages in the treatment of scapular tumor limb salvage. It has light weight, well adapted, relatively simple installation, good histocompatibility, and can obtain a better appearance and function of the shoulder joint after operation. It can become one of the options for limb salvage treatment of scapular tumor.

Clinical application of apatinib in the treatment of advanced bone and soft tissue sar-coma / 中国肿瘤临床

To observe the efficacy and safety of apatinib in the treatment of advanced bone and soft tissue sarcoma, and to analyze the possible related factors affecting the progression-free survival (PFS) of patients. Methods: Twenty-one patients with ad-vanced bone and soft tissue sarcoma admitted to the Department of Orthopaedics, Yunnan Cancer Hospital from June 2017 to Sep-tember 2018, were treated with apatinib tablets. The main efficacy index was progression free survival (PFS), and the secondary effica-cy index was overall survival (OS). Clinical efficacy was evaluated according to response evaluation criteria in solid tumors (RECIST) 1.1, and overall response rate (ORR), disease control rate (DCR), and safety were olserved according to the National Cancer Institute (NCI) 4.0 standard. Results: All of the 21 patients were followed up. At the last follow-up time point, March 31st, 2019, there were no CR, 2 patients (9.5%) with PR, 7 patients with SD (33.3%), and 12 patients with PD (57.1%). The ORR was 9.5%, the DCR was 42.8%, the medi-an PFS was 8 months, and the median OS was 14 months. The patient's gender, age, ECOG score, tissue source, surgery, or chemother-apy had no statistically significant effect on PFS (P>0.05). Only the history of radiotherapy before taking apatinib was a factor for pa-tients with PFS. The effect was statistically significant (P<0.05), and patients with a history of radiotherapy had a lower PFS than pa-tients without a history of radiotherapy. The adverse reactions of gradeⅢand above had hand-foot syndrome (14.3%), pneumotho-rax (14.3%) and anemia (4.8%). Conclusions: Apatinib has a certain effect for advanced bone and soft tissue sarcoma. The adverse re-actions are generally predictable, controllable and reversible. Apatinib can be a choice for patients with advanced bone and soft tissue sarcoma with good treatment adherence and no other treatment options.

The Application of Temporary Balloon Occlusion of the Abdominal Aorta in High-order Position Sacral Tumor Surgical Operation / 昆明医科大学学报

ObjectiveTo evaluate temporary balloon occlusion of the abdominal aorta in high-order position sacral tumor surgical operation as a useful adjuvant technique.MethodsReviewed 79 cases of patients from 2005 to 2015 treated in our department and the diagnosis of high-order position sacral tumor. Temporary balloon occlusion of abdominal aorta was used in 50 patients(male 29,female 21)during the sacral tumors surgical operations. The other 29 patients(male 18,female 11)with sacral tumors who received the non-temporary balloon occlusion therapy were used as control group. The statistical differences of the whole surgery time,the blood loss during the surgery,the happening of the postoperative deep vein thrombosis,the time of the postoperative extubation were analyzed. ResultsThe differences were statistically significant(P<0.001)in the whole surgery time of balloon occlusion group(146.36±29.38)min vs non-balloon occlusion group(206.03±125.93)min,the blood loss of balloon occlusion group(1610.70±491.14)ml vs non-balloon occlusion group(2658.62±562.213)mL, and the time of the postoperative extubation of balloon occlusion group(6.60±2.76)d vs non-balloon occlusion group(12.52±2.86)d. However,there was not significant difference of the happening of the postoperative deep vein thrombosis between balloon occlusion group and non- balloon occlusion group. ConclusionTemporary balloon occlusion of abdominal aorta is effective and reliable. It significantly reduced the time of operations,the loss of blood,mean days in hospital,effusion of post-operation and recurrence rate. It makes the operation of sacral tumors much more safer than before and is a technique worthy of popularizing.

A 2-year follow-up study on 166 leprosy patients treated with uniform multidrug therapy / 中华皮肤科杂志

Objective To assess the efficacy of 6-month uniform multidrug therapy in various types of leprosy. Methods A field trial was conducted among 166 patients with different types of leprosy. All patients were treated with uniform multidrug therapy for 6 months, then followed up for 2 years. Clinical and bacterio-logical improvements were evaluated. Results Among the 166 patients, 31 dropped out due to various reasons,and 135 completed the 6-month treatment and 2-year follow-up. Among the 135 patients, 45 (33.3%) were skin smear negative, and the other smear-positive 90 had an average bacterial index (BI) of 2.91±1.45 (range: 0.1-6.0) before treatment. At the end of the 2-year follow-up, the 45 skin smear-negative patients showed 93.3% improvement in skin lesions and 80.0% improvement in nerve impairments, and the smear-posi-tive 90 patients showed 95.6% improvements in skin lesions and 77.8% improvement in nerve impairments.Skin smear turned negative in 49 (54.4%) out of the smear-positive 90 patients with the average BI declining to 0.66±0.99. The annual decrease in BI reached 0.9 during the first 2.5 years after the beginning of treat-ment. Twenty-five patients developed leprosy reaction during the follow-up, including 13 cases of type Ⅰ leprosy reaction and 12 cases of type Ⅱ leprosy reaction. Relapse was noted in 1 patient with muhibacillary leprosy 13 months after the termination of treatment. Conclusions The short-term efficacy of uniform multidrug therapy is similar to that of 2-year treatment with routine multidrug therapy. However, further studies are required to survey the incidence of leprosy reaction and relapse in patients treated with uniform multidrug therapy.

Rebuilding bone with massive allograft and interlocking intramedullary nail after bone tumor resection / 中国组织工程研究

BACKGROUND: In recent years, the progression of transplantation of allografts has put forward to a new way to repair massive bone and joint defect caused by various reasons, which was a difficult subject in the past.OBJECTIVE: To probe into the effect of massive allograft and interlocking intramedullary nail on rebuilding bone and joint after bone tumor resection.DESIGN: Self-control observation SETTING: Department of Orthopaedics, Yunnan Provincial Tumor Hospital PARTICIPANTS: From April 1998 to January 2003, totally 18 patients with 15 cases of malignant bone tumor and 3 of osteoma-like disease, accepted bone tumor focus removal followed by intrmedullary nailing and allogenic bone transplantation, consented to join the study, were recruited.INTERVENTIONS: Allografts were taken from fresh cadaveric bone (Prepared and stored in Yunnan Provincial Tumor). The bone segment of osteoma was cut completely and the allografts were transplanted at the bone defects. The proximal and distal intrmedullary nailing were inserted in order. Auto cancellous bone particles were transplanted at the bone joint to promote the healing at bone end. The upper part of shoulder bone was transplanted to perform reconstruct of retotar and triangular muscle; half knee joint was transplanted to attach important muscle and ligament. After external fixation of 4 to 6 weeks following operation, the functional exercise was conducted in order sequence. Evaluating the curative effect of functional recovery of the limbs at postoperative 3,6 and 12 months as well as follow-up was carried out according to the criteria of Mankin(excellent ,good , moderate , unsatisfied).MAIN OUTCOME MEASURES: ①Functional recovery of limb of the patients. ② Adverse and side effectRESULTS: Date of totally 18 patients was entered result analysis. ①Functional recovery result of the limbs: According to evaluated criteria put forward by Mankin. In this group, 7 cases of excellent, 6 of good, 4 of moderate and 1 of unsatisfied. Bony callus and the concentration of nuclide were increased gradually in the examination at month 6 and 12. About 10-14 months after operation, the osteotylus would cover the end of allograft, substituted the allograft gradually. ② Adverse events and side effect:In this group, no tumor was survived in 16 cases, two cases died of primary lesion and pulmonary metastasis at postoperative 12 months and 18 months respectively. No infection, bone nonunion, flexible internal fixation or ruptures were found in the limbs of other patients. No one was found with shortened limbs. The patient with part joint transplantation has limitation in joint move, but the joint was stable.CONCLUSION: This operation has the highest stabilization .The patients can do exercise earlier to recover function, and injury arthritis could be prevented. It is suitable to rebuild the bone after bone tumor resection.

The Relation between Obstructive Sleep Apnea Syndrome and Hypertension in Children / 中华高血压杂志

Objective To explore the prevalence of hypertension in children with obstructive sleep apnea syndrome(OSAS).Methods Ninety snoring children were enrolled and stayed in sleeping monitor unit for a night.24 h ambulatory blood pressure(ABP)monitoring and polysomnography were simultaneously carried out. The children were classified depending of [Apnea Hypopnea Index(AHI≥5)] as OSAS group and(AHI

Trends in case detection influenced by leprosy elimination campaigns in certain areas of China.

LECs were carried out from 1998 to 2000 in eight counties of west China. The number of cases detected during the year of LECs was much higher than that detected by routine methods before the year of the LEC. However, the annual number of cases detected during the year after the LEC showed different patterns. One pattern is that the number of new cases detected in the year after the LEC declined to the level similar to that before the year of the LEC. The second pattern is that the number of new cases detected in the year after the LEC declined steeply to less than that detected before the year of the LEC. Following peak case-detection during the year of the LEC, a gradual decrease in the number of new cases was observed in the subsequent years. The repeat LEC brought a weakly rebounding peak case-detection during the year following the first LEC carried out 3 years earlier. The operational, epidemiological and technical factors influencing the trends of case-detection during the LECs are discussed.

A survey of blindness and poor vision in leprosy patients / 中华医学杂志(英文版)

<p><b>OBJECTIVE</b>To determine the prevalence, cause and distributions of blindness and poor vision in patients with leprosy.</p><p><b>METHODS</b>An epidemiological survey of blindness and poor vision among 1045 cases of leprosy was carried out in Taixing City of Jiangsu Province, China.</p><p><b>RESULTS</b>The prevalence of bilateral blindness was 7.67%, unilateral blindness 4.4%, bilateral poor vision of various degrees 9.28% and unilateral poor vision 5.84%. The prevalence of eye complications varied significantly among different groups of patients; females had a higher prevalence than males, multibacillary patients higher than paucibacillary patients, and in-patients higher than out-patients. Corneal disease was the most common cause of blindness in study groups, followed by iritic disease and cataract; while the main cause of poor vision was cataract, then corneal and iritic diseases. Treatable blindness accounted for 62.7% of the cases and treatable poor vision for 88.6% of the patients studied. 56.62% of cases with eye complications expressed their willingness to be treated.</p><p><b>CONCLUSIONS</b>Although prevention and treatment of low vision and blindness in leprosy patients is very hard, it is necessary for doctors and medical workers to make clear of the factors to cause low vision and blindness, especially those in leprosy patients so that some measures for prevention and treatment of the disease could be taken accordingly.</p>

Comprehensive treatment of complicated plantar ulcers in leprosy / 中华医学杂志(英文版)

<p><b>OBJECTIVE</b>To investigate feasible treatment methods for plantar ulcers in leprosy patients according to the agreement between the Ministry of Health (MOH) of China and the Leprosy Mission International (LMI).</p><p><b>METHODS</b>A total of 2599 complicated foot ulcers in 1804 leprosy cases underwent surgic treatment. Plastic fixation and supports were used, dressings were changed regularly, and protective footwear and modified insoles were provided.</p><p><b>RESULTS</b>Of the 2599 foot ulcers 1446 (55.64%) healed. The cure rate of the patients treated in leprosy hospitals was 71.31%, with 219 (15.15%) recurrences of foot ulcers. The recurrence rate of those who lived at home was 18.35%.</p><p><b>CONCLUSIONS</b>Comprehensive treatment of foot ulcers has a high cure rate and a low recurrence rate. Reduction of workload, avoidance of long distance walking, intensification of education on foot self-care and provision of financial support are the main measures for preventing a recurrence of foot ulcers.</p>

Epidemiological study on disabilities in 24128 newly detected leprosy patients in China / 中国康复理论与实践

@#ObjectiveTo approach the status of leprosy disabilities in newly detected cases in recent 11 years in China.MethodsBased upon the records from the National Leprosy Recording and Reporting System in National Center for STD and Leprosy Control, 24128 leprosy cases detected during 1989-1999 in China were analyzed in terms of leprosy disbility.ResultsThe proportion of disabilies in newly detected leprosy cases in 1989 in whole country was 46.49% and decreased to 32.7% in 1999, and the proportions of cases with grade Ⅱ disabilities were 25.55% (1989) and 22.06% (1999). There were 19 provinces where disability rate was more than 40%. Out of patients with disabilities, those with grades Ⅰ and Ⅱ disabilities and with deformities (loss of eyebrow, facial paralysis or saddle nose) accounted for 37.54%, 61.04% and 1.42%, respectively. There were 20 provinces where grade Ⅱ disabilities accounted for more than 50% of all patients with disabilities. The disability rates in patients aged under 15 years, 15-65 years and over 65 years were 24.74%, 39.3% and 53.33%. The patients with a delay in detection of 2 years had a disability rate of 28.95%, and those with a delay of more than 2 years and 5 years had the rates of 48.06% and 60.95%, respectively. The disability rate was 53.76% in patients with leprosy reactions. The grade Ⅱ disability rate in paucibacillary patients (28.53%) was significantly higher than that in multibacillary ones (22.03%). Conclusions Disability rate of leprosy in newly detected cases is still high although it has decreased in the recent 11 years. The rate is associated with delay in detection, leprosy reaction and leprosy type. It suggests that early detection of leprosy patients, regular treatment with multidrug therapy, and management of leprosy reactions will be the effective measures to prevent disabilities of leprosy.

mRNA expression of CD28/B7 molecules in the peripheral blood mononuclear cells of the patients with systemic lupus erythematosus and their significance / 中国免疫学杂志

Objective:To investigate the roles of CD28/B7 molecules expression in the pathogensis of systemic lupus erythematosus and their significance.Methods:Applying reverse transcription polymerase reactin(RT PCR)technique to semiquantitatively analyze CD28?B7 1 and B7 2 mRNA expression in the Peripheral Blood Mononuclear Cells(PBMC)from 35 active SLE patients and 30 cases of healthy subjects.Results:Compared with normal controls,the positive rate of CD28 expression in active SLE patients accounted for 22 86%,which was markedly lower than the normal controls(70 00%),the difference was statistically significant( P

Clinical Analysis of 12 403 Leprosy Relapse Cases in China / 中华皮肤科杂志

Objective To study clinical characteristics and the trends of leprosy relapse cases after dapsone(DDS) monotherapy or multidrug therapy(MDT) from 1949 to 1998. Methods The data of leprosy relapse cases in China from 1949 to 1998 were analyzed. Results There were a total of 12 403 leprosy relapse cases during 1949～ 1998 in China with a cumulative relapse rate of 3.28％ . Among them, 11 803 were relapsed after DDS monotherapy with a relapse rate of 3.83％ and 236 were after MDT with a relapse rate of 0.57％ . The relapse rate (0.84％ ) in PB cases which were previously treated with DDS monotherapy a half year was significantly higher than that (0.36％ ) in PB cases treated with MDT a half year. The relapse rate (0.37％ ) in MB cases which were previously treated with DDS monotherapy plus MDT also significantly higher than that (0.23％ ) in MB cases which were just treated with MDT two years. The proportions of cases with gradeⅡ disabilities and positive skin smears in relapse cases were 49.9％ and 69.3％ , respectively. Conclusion The peak of leprosy relapses after DDS monotherapy occurred during 1959～ 1988. The relapses after MDT started at the end of 1980s and may reach its peak in the next decades.

Studies on Detection Modes of Leprosy in China During the Years 1981 Through 1998 / 中华皮肤科杂志

Objective To analyze the detection modes of leprosy and their influencing factors and outcomes. Methods Based upon the nationwide registration of leprosy patients detected during 1981～ 1998, the profiles of these patients at detection were studied. Results The proportions of cases significantly correlated with calendar years in the detection by dermatologic clinic, contact check, clue survey and mass survey, showing a significant increases in percentage of cases detected through dermatologic clinic and contact check and decreases through clue survey and mass survey. Detection of leprosy cases through dermatologic clinic and voluntary reporting became the main modes of case－ finding during 1997－ 1998, accounting for 37.3％ and 28.6％ respectively, whereas contact check only accounted for 9.1％ . More MB patients were detected by voluntary reporting than PB, whereas detection by dermatologic clinic resulted in a significantly higher proportion (38.0％ ) in PB patients than in MB (33.5％ ). The voluntary reporting and clue survey resulted in higher proportions of detection in prefecture and county rural areas. In areas with good facilities of dermatologic services, significantly higher extent (75.9％ ) of cases were detected through dermatologic clinic, whereas the voluntary reporting and clue survey were main modes of case detection in endemic areas. A great majority of cases were confirmed by leprosy units even though they were detected by various modes. Only 6.5％ of leprosy cases were detected and consequently confirmed by doctors in dermatologic clinics. Conclusion This study emphasizes the importance of providing training on leprosy to the doctors in dermatologic clinics while continuously encouraging their involvement in leprosy control.

Epidemiological Study on Disabilities of Leprosy in Newly Detected Patients in China, 1989－ 1998 / 中华皮肤科杂志

Objective To approach the status of leprosy disabilities in newly detected cases in recent 10 years in China and provide the scientific basis for formulating the preventive strategies. Methods Based upon the individual records from the National Leprosy Recording and Reporting System in National Center for STD and Leprosy Control, 22 437 leprosy cases detected during 1989－ 1998 in China were analyzed in terms of leprosy disability. Results The proportion of disabilities in newly detected leprosy cases in 1989 in whole country was 46.49％ and decreased to 32.31％ in 1998, and the proportions of cases with grade II disabilities were 25.55％ (1989) and 20.23％ (1998). There were 18 provinces where disability rate was more than 40％ . Out of patients with disabilities, those with grades I and II disabilities and with deformities (loss of eyebrow, facial paralysis or saddle nose) accounted for 37.86％ , 60.64％ and 1.5％ , respectively. There were 25 provinces where grade II disabilities accounted for more than 50％ of all patients with disabilities. The disability rates in patients aged under 15 years, 15－ 65 years and over 65 years were 24％ , 39.85％ and 53.33％ . The patients with a delay in detection of 2 years had a disability rate of 29.85％ , and those with a delay of more than 2 years and 5 years had the rates of 48.82％ and 61.17％ , respectively. The disability rate was 52.9％ in patients with leprosy reactions and 46.1％ in patients with a damage of more than 3 nerves. The grade II disability rate in paucibacillary patients(28.99％ ) was significantly higher than that in multibacillary ones (22.04％ ). Conclusion Disability rate of leprosy in newly detected cases is still high although it has decreased in the recent 10 years. The rate is associated with delay in detection, leprosy reaction and leprosy type. It suggests that early detection of leprosy patients, regular treatment with multidrug therapy, and management of leprosy reactions will be the effective measures to prevent disabilities of leprosy.

Evaluation of Effectiveness in implementing Leprosy Elimination Campaigns in High Leprosy- Epidemic Areas in Yunnan, Guizhou and Sichuan Provinces / 中华皮肤科杂志

Objective To evaluate the effectiveness of Leprosy Elimination Compaigns (LEC) integrated with routine case- finding of leprosy in the high leprosy- epidemic areas in Yunnan, Guizhou and Sichuan Provinces. Methods Extensive health education of leprosy in the community, training of paramedical workers on leprosy, clue survey and contact survey were carried out to make diagnosis and provide treatment. Results A total of 122 new leprosy cases were detected in 6 high leprosy- epidemic counties through LEC in 1999 with a detection rate of 3.7/100 000, but in contrast, the annual number of newly detected leprosy cases was 67 cases with an average detection rate of 2/100 000 during the period of 3 years before LEC. Among the newly detected leprosy cases, about 47％ were leprosy household contacts. Conclusion LEC combined with routine activities of case finding can improve the detection of the back- log cases in the communities. In the high epidemic areas, especially in the remote and difficult- to- access areas, majority of leprosy cases can be detected through combining active and passive case- finding methods, providing extensive health education, improving leprosy services in skin clinic, leprosy contact and clue surveys.

Prediction of Elimination of Leprosy in Leprosy Endemic Areas of China / 中华皮肤科杂志

Objective To have insight into the epidemiological situation and to predict the possibilities for achieving the national goal of basic eradication or the WHO target of elimination of leprosy as a public health in the leprosy high－ endemic areas in China. Methods Based upon the data during 1983－ 1996 from the National Leprosy Recording and Reporting Surveillance System and using the appropriate mathematical models, the expected calendar year of reaching the national goal in terms of detection and prevalence rates and the WHO target of elimination under different conditions was predicted. Results Of 337 counties where the national goal of basic eradication of leprosy had not reached and 40 counties where the WHO goal of leprosy elimination had not achieved in 1996, the detection rates in calendar years followed the negatively exponential models with a significant goodness－ of－ fit in 67 and 11 counties, respectively. In the former, the national goal can be met in 6％ of counties before the year 2000 or 34.4％ before 2010 in terms of detection rate, or 31.3％ before the year 2010 in terms of prevalence rate. In the latter, the WHO target can be met in 8－ 10 counties within this century when the duration of disease was determined with the WHO definition. While the MB proportion among new cases increased by 10％ , the target would be met one year later. However, at the same MB proportion, the change of the duration of completing the fixed treatment from PB 0.5 year and MB 2 years to PB 0.75 year and MB 3 years will result in the achievement of the goal 2－ 10 years later. Conclusion The results imply that WHO goal of leprosy elimination can be reached in more than 95％ of counties by the end of this century, but the national goal of achieving basic eradication of leprosy in more than 95％ of counties by this century will not be met, indicating that leprosy control will go beyond the century in China.

Levels of Th2 cytokine receptors in the peripheral blood mononuclear cells of the patients with lupus erythematosus and its clinical significance / 中国免疫学杂志

Objective:To investigate the roles of Th2 cytokine receptors in the pathogenesis of systemic lupus erythematosus(SLE) and its clinical significance.Methods:RT-PCR technique was used to semiquantitatively analyze Th2 cytokine receptors mRNA expression in PBMC from 89 patients with SLE,double antibody sandwich ELISA was applied to determine Th2 cytokines in serum.Results:The positive Th2 cytokine receptors mRNA expression accounted for 100% among active SLE group(group Ⅰ),inactive SLE group(group Ⅱ) and control group(group Ⅲ).The mean levels of IL-4R mRNA expressions in group Ⅰ,Ⅱ and Ⅲ were 0.604?0.147,0.40?0.13 and 0.37?0.07,respecitively.Compared with group Ⅱ and group Ⅲ,the mean level of IL-4R mRNA expressions in group Ⅰ was markedly increased(P=0.00),while there were no statistical differences between group Ⅱ and group Ⅲ(P=0.351).The mean levels of IL-6R mRNA expressions in group Ⅰ,Ⅱ and Ⅲ were 0.902?0.273,0.519?0.11 and 0.573?0.24,respectively.Compared with group Ⅱ and group Ⅲ,the mean level of IL-6R mRNA expressions in group Ⅰ was markedly increased(P=0.00),while there were no statistical differences between group Ⅱ and group Ⅲ(P=0.289).The mean levels of IL-10R mRNA expressions in group Ⅰ,Ⅱ and Ⅲ were 0.87?0.29,0.722?0.209 and 0.684?0.138,respectively.Compared with group Ⅱ and group Ⅲ,the mean level of IL-10R mRNA expressions in group Ⅰ was markedly increased(P=0.00),while there were no statistical differences between group Ⅱ and group Ⅲ(P=0.405).The mean levels of IL-4 in serum in group Ⅰ,Ⅱ and Ⅲ were 105.57?29.17,51.17?16.69 and 23.73?6.24,respectively.Compared with group Ⅱ and group Ⅲ,the mean level of IL(4 in group Ⅰ was significantly higher than those in group Ⅱ and group Ⅲ(P=0.00),while that in group Ⅱ was higher than group Ⅲ(P=0.00).The mean levels of IL-6 in serum in group Ⅰ,Ⅱ and Ⅲ were 71.89?20.02,17.96?6.93 and 0.035?0.035,respectively.Compared with group Ⅱ and group Ⅲ,the mean level of IL-6 in group Ⅰ was significantly higher than those in group Ⅱ and group Ⅲ(P=0.00),while that in group Ⅱ was higher than group Ⅲ(P=0.00).The mean levels of IL-10 in serum in group Ⅰ,Ⅱ and Ⅲ were 32.32?11.51,18.66?6.63 and 0.289?0.867,respectively.Compared with group Ⅱ and group Ⅲ,the mean level of IL-10 in group Ⅰ was significantly higher than those in group Ⅱ and group Ⅲ(P=0.00),while that in group Ⅱ was higher than group Ⅲ(P=0.00).There were positive correlation between IL-4 and IL-4R in active group and inactive group(r=0.622-0.859,P

Polymorphism within the interleukin-10 receptor cDNA gene in patients with systemic lupus erythematosus / 中华皮肤科杂志

Objective To assess the association between polymorphism within the interleukin-10 receptor cDNA gene (IL-10R) and Chinese patients with systemic lupus erythematosus (SLE). Methods The IL-10R genotypes of 94 SLE patients and 80 healthy subjects were examined by reverse transcription-polymerase chain reaction-single strand conformation polymorphism method (RT-PCR-SSCP), RT-PCR-restriction fragment length polymorphism (RFLP) and DNA sequencing. Results There were significant differences in the IL-10R2 genotype frequencies of these two groups. The IL-10R2 G520/G520 genotype increased the risk of developing SLE (OR = 0.515, 95% CI 0.414-0.579, P = 0.004) and individuals who had G520/A520 genotype also had a higher susceptibility to SLE (OR = 1.968, 95% CI 0.981-3.949, P = 0.055). There was no significant association between SLE and IL-10R1 genotypes. The risk of developing SLE was detected in the individuals who had the combination of IL-10R1 G241/G241 and IL-10R2 G520/G520 (OR = 0.515, 95% CI 0.444-0.597, P = 0.004). Conclusion The IL-10R2 genotypes of G520/G520 and G520/A520 as well as the combination of genotypes IL-10R1G241/G241 and IL-10R2 G520/G520 may increase the susceptibility to SLE in Chinese people.

IL-6 Receptor Expression in Peripheral Blood Mononuclear Cells of the Patients with Lupus Erythematosus and its Clinical Significance / 中华皮肤科杂志

Objective To investigate the role of IL-6 and IL-6R in the pathogenesis of systemic lupus erythematosus (SLE) and its clinical significance. Methods RT-PCR technique was used to semiquantitatively analyze IL-6R mRNA expression in peripheral blood mononuclear cells (PBMC), and double antibody sandwich ELISA was applied to determine the serum level of IL-6 from 89 SLE patients . Results The positive IL-6R mRNA expression was found in each individuals among active SLE group (groupⅠ), inactive SLE group (groupⅡ) and control group (group Ⅲ). The mean levels of IL-6R mRNA expressions in groupⅠ,Ⅱ or Ⅲ were 0.902 ? 0.273, 0.519?0.11 and 0.573?0.24, respectively. Compared with group Ⅱand group Ⅲ, the mean level of IL-6R mRNA expression in groupⅠ was markedly increased (P = 0.00), while there was no statistical difference between group Ⅱ and group Ⅲ(P = 0.289). The mean serum levels of IL-6 in groupⅠ,Ⅱ and Ⅲ were 71.89 ? 20.02,17.96 ? 6.93, and 0.035 ? 0.035, respectively. Compared with group Ⅱand group Ⅲ, the mean level of IL-6 in groupⅠ was significantly higher than those in groupⅡ and groupⅢ (P = 0.00), while that in groupⅡwas higher than group Ⅲ (P = 0.00). There was a positive correlation between IL-6 and IL-6R in active SLE group and inactive SLE group(r = 0.887, and r = 0.615 P

Activity of Home-made Ofloxacin Against M. leprae in Mice and its Clinical Trial in Multibacillary Leprosy / 中华皮肤科杂志

Seventy days after inoculation of 1. 0 ? 104 M. leprae to the foot--pads,the mice were treated with home-made ofloxacin 150mg/kg daily by gavage 5 times weekly for 63 days. The results showed that M. leprae multiplied well in the foot--pads of untreated mice,but no growth of M. lepare were found in the foot-pads of the mice treated with home-made ofloxacin, imported ofloxacin or rifampin until 12 months after infection of M. leprae. Eleven newly diagnosed and untreated BL/LL patients and 2 recently relapsed patients after dapsone monotherapy were treated with home--made ofloxacin 400mg daily 6 times weekly for 12 weeks. The results showed that the skin lesions of all patients markedly improved at the end of therapy. The mean morphological index(MI) in skin smear decreased from 11. 89% before therapy to 2. 59% at the end of 2 week treatment,and to 0% at the end of 4 week treatment. The mean bacteriological index(MI) decreased from 4. 28 before therapy to 3. 90 at the end of therapy. During the therapy, 4 patients developed type Ⅱ lepra reaction, but all patients tolerated the drug well. The study showed that the therapeutic effect of home-made ofloxacin is similar to that of imported one as reported in the literature. The home-made ofloxacin can be used in multidrug therapy for leprosy.